Sponsored Talks
Nanion Technologies GmbH
Speaker: Fitzwilliam Seibertz (University of Göttingen, DE)
Symposium by the LS2 Section Ion Channels and Membrane Transporters: Understanding the Role of Ion Channels and Membrane Transporters in Cardiac and Renal Diseases - 17 Feb 2023 at 10:20 - 12:05
Title: APC and the atria: High performance automated patch clamp of mammalian atrial cardiomyocytes.
Abstract: Atrial fibrillation (AF) is the most common cardiac arrhythmia reported in the clinics. Current AF therapeutics lack efficacy, and mechanistic strategies to examine ion channel remodelling in AF are limited by a lack of atrial specificity in expression systems or low throughput methods. Much progress has been made in the development of automated patch-clamp (APC) systems that allow for high throughput electrophysiological measurements. APC could therefore be a useful tool for increasing the throughput of electrical investigations into AF mechanisms.
We describe the application of the high throughput APC device (Nanion SyncroPatch 384) to investigate key currents and action potentials (AP) in human atrial-specific iPSC-derived cardiomyocytes (iPSC-CM), and in atrial cardiomyocytes directly isolated from native swine myocardium. We describe successful current and AP acquisition from native mammalian cardiomyocytes using APC. We observed typical subtype-specific electrophysiological characteristics including a shorter AP, smaller L-type calcium current (ICa,L), and smaller inward rectifier potassium current (IK1) in atrial cells compared to ventricular. In addition, activation of the atrial-specific acetylcholine-activated inward rectifier potassium current (IK,ACh) was exclusively observed in atrial but not in ventricular cells following application of the M-receptor agonist carbachol.
The successful application of a high throughput APC-system for the recording of atrial APs and ionic currents in freshly isolated mammalian cardiomyocytes implies that APC represents a crucial tool for increasing the throughput of electrophysiological measurements of mammalian cardiac tissue. This could facilitate robust studies of AF mechanisms and substantially impact AF-related drug development programs.
Bruker Switzerland AG
Speaker: Torsten Müller
Title: On the benefits and challenges of single cell proteomics
Symposium by the LS2 Section Proteomics: Single Cell Proteomics: Are We There Yet? - 17 Feb 2023 at 14:05 - 15:50
