Sponsored Talks
Olivier Buetzberger (Agilent Technologies)
Title: Real-Time Cellular Metabolism Analysis: Agilent Seahorse Analyzers and Integrated Microscopy for Comprehensive Mitochondrial and Glycolytic Function Profiling
Mitochondrial function and glycolysis play critical roles in a variety of vital cellular processes, including cellular activation, proliferation, differentiation, cell death, and disease progression. Agilent offers solutions that enable the measurement of metabolic parameters and functions using live cells, in real-time, in a microplate. The Agilent Seahorse Analyzers profile cellular metabolic functions, using label-free, solid-state disposable optical sensors. Seahorse simultaneously measure mitochondrial respiration via the oxygen consumption rate (OCR), and glycolysis via the extracellular acidification rate (ECAR). Seamless integration with the Agilent BioTek Cytation 5 allows easy and accurate normalization through imaging. Assay kits and reagents provide standard methods for quantifying mitochondrial respiration, glycolytic activity, endogenous and exogenous fatty acid oxidation, substrate oxidation, and metabolic phenotype. In addition, Agilent’s soluble sensors offer a simple kinetic measurement of aerobic metabolism that can be performed in microplate format using fluorescence plate readers such as the Agilent BioTek Synergy H1. These technologies have been applied to multiple research areas, including cancer, obesity, diabetes, metabolic disorders, immunology, cardiovascular function, neurodegeneration, virology, and aging.
